Journal: Molecular Vision

Article Title: Increased pro-MMP9 plasma levels are associated with neovascular age-related macular degeneration and with the risk allele of rs142450006 near MMP9

doi:

Figure Lengend Snippet: Subjects used for TIMP3 measurements.

Article Snippet: TIMP3 levels were quantified using the Human TIMP-3 ELISA Kit (Cat. Nr: EA100363; OriGene Technologies Inc., Rockville, MD).

Techniques:

Journal: Molecular Vision

Article Title: Increased pro-MMP9 plasma levels are associated with neovascular age-related macular degeneration and with the risk allele of rs142450006 near MMP9

doi:

Figure Lengend Snippet: TIMP3 levels in plasma samples of nAMD patients and controls. A : Comparison of TIMP3 levels in nAMD patients and controls. B : TIMP3 levels in nAMD patients and controls mixed stratified by rs5754227 genotype. C : TIMP3 levels in nAMD patients and controls separately and stratified by rs5754227 genotype. The non-risk allele of rs5754227 is depicted in green. Bars represent median values, and whiskers represent interquartile ranges.

Article Snippet: TIMP3 levels were quantified using the Human TIMP-3 ELISA Kit (Cat. Nr: EA100363; OriGene Technologies Inc., Rockville, MD).

Techniques:

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: Concentrations of TIMP3, MMP3, HE4 and CA125 in blood serum, depending on the histopathologic diagnosis of patients

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques: Biomarker Discovery

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: The ROC curve for MMP3, TIMP3, HE4 and CA125 protein in diagnostic patients with ovarian cancer and with benign ovarian cysts without hormonal status division

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques: Diagnostic Assay

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: The ROC curve for MMP3, TIMP3, HE4 and CA125 protein in diagnostic patients with ovarian cancer and with benign ovarian cysts for postmenopausal women

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques: Diagnostic Assay

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: Individual proteins sensitivity and specificity depending on hormonal status

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques:

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: Cox regression analyses of progression free survival and overall survival of classic prognostic factor, MMP3 and TIMP3

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques:

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: Individual proteins concentration levels in patients with ovarian cancer, depending on the staging and grading

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques: Concentration Assay

Journal: Journal of Ovarian Research

Article Title: Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer

doi: 10.1186/s13048-017-0373-9

Figure Lengend Snippet: Overall survival stratified by MMP3 and TIMP3 in examined patient with ovarian cancer

Article Snippet: TIMP3 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, USA) using commercial Human TIMP3 Magnetic Bead Panel 2 (Merck Millipore, Billerica, MA, USA).

Techniques:

Journal: bioRxiv

Article Title: Regulatory mechanisms in multiple vascular diseases locus LRP1 involve repression by SNAIL and extracellular matrix remodeling

doi: 10.1101/2023.05.09.539992

Figure Lengend Snippet: A : Illustration of indel frameshift in exons of LRP1 to generate knockout iPSCs using CRISPR/Cas9 prior to differentiation into SMCs B: Representative Western blot images showing LRP1 protein expression in WT and LRP1 KO iPSC-SMCs. C : Heatmap representation of relative RNA expression for differentially expressed genes between LRP1 KO and WT iPSC-SMCs. Genes involved in “collagen containing extracellular matrix” (gene ontology 0062023) are indicated D : Representative Western blot images showing the expression of phospho-SMAD2/3 (p-SMAD2/3) and total SMAD2/3 (SMAD2/3) in WT and LRP1 KO iPSC-SMCs in the presence of TGFβ1 protein for 1 hour. E : Heatmap representation of label free quantification (LFQ) for proteins over or underrepresented in extracellular extracts of LRP1 KO and WT iPSC-SMCs. F : Representative Western blot images showing the expression of LRP1, CYR61 and TIMP3 in whole cell extracts or decellularized extracts (ECM).

Article Snippet: Following antibodies were used in Western blot experiments: rabbit anti-LRP1 (ab92544, Abcam, Cambridge, UK), mouse anti-β-actin (sc-47778, Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-phospho-SMAD2/3 (#8828) and anti-SMAD2/3 (#3102, Cell Signaling Technology, Danvers, Massachusetts, USA), mouse anti-TIMP3 (LS-B2576, Lifespan Biosciences, Seattle, Washington, USA), mouse anti-CYR61 (sc-374129, Santa Cruz Biotechnology, Dallas, Texas, USA).

Techniques: Knock-Out, CRISPR, Western Blot, Expressing, RNA Expression